Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes.

نویسندگان

  • Guorui Li
  • Qin Liang
  • Ping Gong
  • Adam H Tencer
  • Zhihao Zhuang
چکیده

We report a new class of deubiquitinating enzyme (DUB) probes that resemble the native diubiquitin with a same linkage size and contain a Michael addition acceptor for trapping the DUB active-site cysteine. Both K63- and K48-linked diubiquitin probes were generated using a facile chemical ligation method. The diUb probes were demonstrated to label DUBs from different families and revealed intrinsic linkage specificities of DUBs.

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عنوان ژورنال:
  • Chemical communications

دوره 50 2  شماره 

صفحات  -

تاریخ انتشار 2014